bdhA [2017-03-31 14:40:38]

acetoine/ butanediol dehydrogenase

locus: BSU06240

pI: 4.80

mw: 37.19 kDa

protein length: 346 aa

gene length: 1038 bp

function: overflow metabolism, fermentation

product: acetoine/ butanediol dehydrogenase

essential: no

synonyms: ydjL

Genomic Context

categories

• [category|SW 2|Metabolism] → [category|SW 2.2|Carbon metabolism] → [category|SW 2.2.1|Carbon core metabolism] → [category|SW 2.2.1.5|Overflow metabolism]
• [category|SW 6|Groups of genes] → [category|SW 6.4|Phosphoproteins] → [category|SW 6.4.1|Phosphorylation on an Arg residue]

This gene is a member of the following [SW|regulons]

• [SW|AbrB regulon]

Gene

Coordinates: 677,911 -> 678,951

The protein

Catalyzed reaction/ biological activity

• formation of butanediol from acetoin [Pubmed|18820069]

Modification

• phosphorylated on Arg-13 [Pubmed|22517742]

Structure

• [PDB|4EJ6] (from ''Sinorhizobium meliloti'', 33% identity)

additional information

• belongs to the 100 [SW|most abundant proteins] [PubMed|15378759]

Expression and Regulation

Operons

• genes: [gene|37A9CD057DC311397B9A5757F6ED7AE6998F6D5D|bdhA]
  description: [Pubmed|22383849]

  regulatory mechanism

  • [protein|E5110D9C36E8C55AFD1419987B14A53232165F20|AbrB]: repression, [Pubmed|20817675], in [regulon|E5110D9C36E8C55AFD1419987B14A53232165F20|AbrB regulon]

Biological materials

Mutant

• MGNA-C219 (ydjL::erm), available at the [https://shigen.nig.ac.jp/bsub/resource/strainGeneDisrupted/detail/2217 NBRP B. subtilis, Japan]

two-hybrid system

• ''B. pertussis'' adenylate cyclase-based bacterial two hybrid system ([SW|BACTH]), available in [SW|Jörg Stülke]'s lab

References

• 18820069,22361854,22517742,22178965,20817675,23576037,15378759,24608678,26385762,26825987